(2022.02.01 - now) Assistant Professor, Department of Biomedical Engineering, Chang Gung University, Taiwan
(2022.08.01 - now) Adjunct assistant research fellow, Department of Neurosurgery, Chang Gung Memorial Hospital Keelung, Taiwan
Advisor, Ryudyn co Ltd., Taiwan
(2014 - 2020) Ph.D., Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan
(2010 - 2012) M.S., Institute of Biophotonics, School of Biomedical Science and Engineering, National Yang-Ming University, Taipei, Taiwan
(2006 - 2010) B.S., Clinical Laboratory Science and Medical Biotechnology, School of Medicine, National Taiwan University, Taipei, Taiwan
2007 - 2010, thematic research in Teng's Group, Clinical Laboratory Science and Medical Biotechnology, National Taiwan University, Taiwan
Advisor: Prof. Teng, Lee-Jene
Research Topic: The Effect of Farnesol on the Lipase and Protease Activity of Staphylococcus aureus and Staphylococcus epidermidis
2010 - 2012, graduate study & research assistant in BioMicrofluidic Applications Laboratory (BioMAps), Research Center for Applied Sciences, Academia Sinica, Taiwan
Advisor: Research Fellow, Dr. Cheng, Ji-Yen
Thesis Title: Electric Field Directed Lung Adenocarcinoma Cell Migration in a Micro-Controlled Environment
2012 - 2014, thematic research under Dr. Cheng, Ji-Yen, Academia Sinica
Research Topic: Microfluidic cell culture platform
Research project: Whole cell sensing platform
2014 - 2020, Graduate Research Assistant, Micro/Bio/Nanofluidics Unit in Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan
Topic: Automated microfluidic platform for cell biology and bioengineering research.
Thesis title: Glioma-on-Chips: Analysis of glioma cell guidance and interaction in microfluidic-controlled microenvironments enabled by machine learning
2017 - 2020, DC1 Research Fellow for JSPS, Micro/Bio/Nanofluidics Unit in Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan
Topic: Study of glioma infiltration in complex microenvironment on an automated microfluidic chip.
2020.2 - 2020.3, PD Research Fellow for JSPS, Micro/Bio/Nanofluidics Unit in Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan
2020.3 - 2020.7, Junior Research Fellow, Micro/Bio/Nanofluidics Unit in Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan
2020.8 - 2021.12, Technology Pioneer Fellow, TDIC, Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan
Fellowship, award, and certificate
2008 National Taiwan University Department of Clinical Laboratory Science and Medical Biotechnology Theodore Memorial Scholarship
2009 Research Creativity Award from the National Science Council of Taiwan.
Medical Technologist, Taiwan (2011)
2012 National Yang-Ming University Annual Thesis Competition Excellent Award
Emergency Medical Technician-1, Taiwan (2014)
Oral Presentation, International Photonics Conference 2011 (IPC2011) (Dec.8-10, 2011, NCKU, Tainan, Taiwan)
Poster, The 20th Symposium on Recent Advances in Cellular and Molecular Biology (Feb.7-9, 2012, Kaohsiung, Taiwan)
Poster, The 17th Biophysics Conference, (May 23-25, 2012, Taipei, Taiwan)
Poster, The 4th International Symposium on Microchemistry and Microsystems (ISMM 2012), (Jun.10-13, 2012, Zhubei city, Hsinchu, Taiwan)
Poster, The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012), (Oct.28-Nov.1, 2012, Okinawa, Japan)
Poster, Advances in Microfluidics and Nanofluidics 2014 (AMN2014), (May, 2014, Academia Sinica, Taipei, Taiwan)
Technical presentation, NanoEngineering for Medicine and Biology 2016 (NEMB2016) (Feb, 2016, Houston, Texas, USA)
Poster presentation, Lab on chip world congress 2017. (Oct, 2017, California, USA)
Poster presentation, Lab on Chip & Microfluidics Europe 2019 (Jun, 2019, Rotterdam, The Netherlands)
Total citation:285; h-index:7 (Scopus)
Total citation:389; h-index:8 (Google Scholar citations)
Hsieh-Fu Tsai*, Daniel W. Carlson, Anzhelika Koldaeva, Simone Pigolotti and Amy Shen*, Optimization and Fabrication of Multi-Level Microchannels for Long-Term Imaging of Bacterial Growth and Expansion, Micromachines, 13(4), 576 (2022)
[Summary] A straightforward microfabrication process for multi-level microfluidic devices using maskless writer. We validated the hydrodynamics performance of the microfluidic chip by both finite element and finite volume methods (COMSOL Multiphysics and openFOAM). Although it is known that the mineral oil dries over time and the oil on oil immersion oil has to be cleaned up, to our knowledge it is the first report that drying of oils increase it's viscosity over time but not refractive index. Certain oils with increased viscosity due to aging caused ghosting effects and slowly drift away from the desired focus over time, limiting the total duration of live bacterial imaging.
Anzhelika Koldaeva, Hsieh-Fu Tsai, Amy Q. Shen and Simone Pigolotti*, Population genetics in microchannels, PNAS, 119(21), e2120821119 (2022)
[Summary] Study of bacterial expansion dynamics in microfluidic open-ended devices that are fabricated by one-station maskless lithography. The bacteria forms defined lanes through competition with each other. The single organisms in the middle of the channel has higher probabilities to stay longer throughout the lineage.
Hsieh-Fu Tsai, Camilo IJspeert, Amy Q. Shen*, Voltage-gated ion channels mediate the electrotaxis of glioblastoma cells in a hybrid PMMA/PDMS microdevice, APL Bioengineering, 4, 036102 (2020)
[Emerging SCI, EI]
[Summary] By using a hybrid PMMA/PDMS microdevice to study the electrotaxis of glioblastoma. We identified that T98G and U-251MG glioblastoma cell lines has opposite electrotactic and electroalignment behavior. Also, the electrotaxis of glioblastoma cell lines depends on the laminin extracellular matrix, which in the brain is exclusively associated with the vasculature, suggesting a link to the peri-vascular microenvironment. The role of voltage gated calcium channels are further investigated in the electrotaxis of glioblastoma cells.
Hsieh-Fu Tsai*, Kazumi Toda-Peters, Amy Q. Shen*, Glioblastoma adhesion in a quick-fit hybrid microdevice, Biomedical Microdevices, 21(2), 30, (2019)
[Summary] A novel quick-fit hybrid PMMA/PDMS microdevice is developed and proved by studying a glioblastoma adhesion model. Over 150 V/m electric field, the electroalignment effect modulated the parallel alignment of the shear flow on the endothelial cells. We also observed the intercellular transport event suggesting that indeed cancer cells and fuse with endothelial cells.
Hsieh-Fu Tsai*, Joanna Gajda, Tyler F.W. Sloan, Andrei Rares, Amy Q. Shen*, Usiigaci: Instance-aware cell tracking in stain-free phase contrast microscopy enabled by machine learning, SoftwareX, 9, 230-237 (2019)
[Original software article]
[Summary] Usiigaci is an all-in-one Python-based cell segmentation, tracking, data analysis pipeline to analyze cell migration under stain-free phase contrast microscopy using machine learning methods (Mask R-CNN).
Hsieh-Fu Tsai, Alen Trubelja, Amy Q. Shen* and Gang Bao*, Tumour-on-a-chip: microfluidic models of tumour morphology, growth and microenvironment, Journal of Royal Society Interface, 20170137 (2017)
[Summary] Review some recent microfluidic platforms targeting research in tumor microenvironments and applications.
Toshiaki Mochizuki, Yi-Jyun Luo, Hsieh-Fu Tsai, Akane Hagiwara and Ichiro Masai*, Cell division and cadherin-mediated adhesion regulate lens epithelial cell movement in zebrafish, Development, 144, 708-719 (2017)
[Summary] Investigation of cadherin and mechanical force in regulating lens epithelial cell movement during early development of zebrafish lens. I was involved specifically in the experiment of UV laser ablation and observe the immediate force induced cell migration after a wound.
Joshua J. Cardiel, Daisuke Takagi, Hsieh-Fu Tsai and Amy Q. Shen*, Formation and flow behavior of micellar membranes in a T-shaped microchannel, Soft Matter, 12, 8226-8234 (2016)
[Summary] Measurement and characterization of wormlike micelle membrane formation when interacting at the interface in the T-channel.
Hsieh-Fu Tsai, Ji-Yen Cheng, Hui-Fang Chang, Tadashi Yamamoto, Amy Q. Shen*, Uniform electric field generation in circular multi-well culture plates using polymeric inserts, Scientific Reports, 6:26222 (2016)
[Summary] It is very difficult to create uniform electric fields from two electric potentials in a circular chamber due to differences of electric resistances. We employed a rational 3D computer aided design (CAD) approach of a microfluidic structure to equalize the electrical resistances in the chamber. A polymer insert with CAD structures can create uniform electric field in wells of multi-well plates or tissue culture dishes very much like a transwell insert. We hope this design of insert can be useful to push electric field stimultion into standard biomedical laboratory. Also the design strategy could be useful for different disciplines.
Hsieh-Fu Tsai, Yi-Ching Tsai, Sharon Yagur-Kroll, Noa Palevsky, Shimshon Belkin, Ji-Yen Cheng*, Water pollutant monitoring by a whole cell array through lens-free detection on CCD, Lab on a Chip, 15, 1472-1480 (2015)
[Summary] Created a miniaturized platform for bioluminescent whole cell sensor using commercial linear charge-coupled devices.
Hsien-San Hou, Hsieh-Fu Tsai, Hsien-Tai Chiu, and Ji-Yen Cheng*, Simultaneous chemical and electrical stimulation on lung cancer cells using a multichannel-dual-electric-field chip, Biomicrofluidics, 8, 052007 (2014)
Kai-Yin Lo, Yun Zhu, Hsieh-Fu Tsai, and Yung-Shin Sun, Effects of shear stresses and antioxidant concentrations on the production of reactive oxygen species in lung cancer cells, Biomicrofluidics, 7, 064108 (2013)
Hsieh-Fu Tsai, Ching-Wen Huang, Hui-Fang Chang, Jeremy J.W. Chen, Chau-Hwang Lee, Ji-Yen Cheng*, Evaluation of EGFR and RTK signaling in the electrotaxis of lung adenocarcinoma cells under dcEF stimulation, PLoS ONE, 8(8), e73418 (2013)
[Summary] found that CL1-5 lung adenocarcinoma cell electrotaxis is independent of EGFR signalling despite CL1-5 cells express an abundant amount of EGFR. Suggesting the fundamental hypothesis may required modification.
Hsieh-Fu Tsai, Shih-Wei Peng, Chun-Ying Wu, Hui-Fang Chang, Ji-Yen Cheng*, Electrotaxis of oral squamous cell carcinoma cells in a multiple-electric-field chip with uniform flow field, Biomicrofluidics, 6, 034116 (2012)
Supplementary Information difficult to get from AIP, if supplementary information is needed please contact.
[Summary] multiple electric field with uniform flow field creation based on R-2R ladder network concept. Also found a easy method to achieve bubble free cell culture in thermoplastic microfluidic chips.
Ji-Yen Cheng*, Mansoureh Z. Mousavi, Chun-Ying Wu, Hsieh-Fu Tsai, Blue light emission from a glass/liquid Interface for real-time monitoring of a laser-induced etching process, Journal of Micromechanics and Microengineering, 21, 075019 (2011)
2010 - 2010, Internship, National Taiwan University Hospital Clinical Laboratory, Taiwan
2013 - 2014, Alternative Civilian Service in Ministry of Health and Welfare, Taiwan
2014 - 2014, Research assistant to Dr. Cheng, Ji-Yen, Academia Sinica, Taiwan
2020.3 - 2020.7, Junior Research Fellow, Okinawa Institute of Science and Technology, Okinawa, Japan
2020.8 - 2021.12, Technology Pioneer Fellow, TDIC, Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan
2021.09 - now, Founder, Ryudyn corporated limited, Taiwan
2022.02 - now, Advisor, Ryudyn corporated limited, Taiwan
EP3350310B1 3D POLYMERIC INSERT TO APPLY UNIFORM ELECTRIC FIELD IN CIRCULAR CULTUREWARE
US10851337B2 3D POLYMERIC INSERT TO APPLY UNIFORM ELECTRIC FIELD IN CIRCULAR CULTUREWARE
US provisional application 62/968,768 A DETACHABLE MICROFLUIDIC SYSTEM AND METHOD FOR ROBUST SPARSE CELL SEEDING WITHOUT BUBBLES.
US Provisional Patent Application No. 63/287,005 SYSTEM AND METHODS FOR OBJECT IMAGING, REAL-TIME ANALYSIS, AND PHENOTYPIC PREDICTION IN A MODULAR MICROSCOPE EMBODIMENT WITH INTERNET INTERACTIVITY
2008 - 2009 Undergraduate Student Thematic Grant from National Science Council of Taiwan, Grant No.NSC97-2815-C-002-072-B
Grant topic: The Effect of Farnesol on the Lipase and Protease Activity of Staphylococcus aureus and Staphylococcus epidermidis. (TWD$48000)
2017 - 2020 Grands-in-Aid for Scientific Research (KAKENHI) to Research Fellowship for Young Scientists (DC1) from Japan Society for the Promotion of Science (JSPS), Japan
Grant topic: Study of glioma infiltration in complex microenvironment on an automated microfluidic chip. (17J00362) (¥3.1M)
2020 - 2021 Proof-of-Concept Grant awarded to Technology Pioneer Fellow, OIST
A compact AI cytoscope for label-free prediction and tracking of cell phenotype and behavior
2022-2023 Individual Research Grant from National Science and Technology Council of Taiwan, Grant No. NSTC 111-2222-E-182-004
Grant topic: 開發多重力場擬器官微流體晶片用以探索腦癌血管微環境中力學拉伸對神經膠細胞和內皮細胞與神經膠質母細胞瘤細胞互動之影響
Development of multiplex force-field organ-on-a-chip for exploring mechanical stimulation on glial cells and endothelial cells and their interactions with glioblastoma cells in mimicked brain cancer microvascular environment.