Curriculum Vitae

Affiliation

  • Assistant Professor, Department of Biomedical Engineering, Chang Gung University, Taiwan

Education

(2006 - 2010) B.S., Clinical Laboratory Science and Medical Biotechnology, School of Medicine, National Taiwan University, Taipei, Taiwan

(2010 - 2012) M.S., Institute of Biophotonics, School of Biomedical Science and Engineering, National Yang-Ming University, Taipei, Taiwan

(2014 - 2020) Ph.D., Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan

Research experience

  • 2007 - 2010, thematic research in Teng's Group, Clinical Laboratory Science and Medical Biotechnology, National Taiwan University, Taiwan

          • Advisor: Prof. Teng, Lee-Jene

          • Research Topic: The Effect of Farnesol on the Lipase and Protease Activity of Staphylococcus aureus and Staphylococcus epidermidis

          • Report link

  • 2010 - 2012, graduate study & research assistant in BioMicrofluidic Applications Laboratory (BioMAps), Research Center for Applied Sciences, Academia Sinica, Taiwan

          • Advisor: Research Fellow, Dr. Cheng, Ji-Yen

          • Thesis Title: Electric Field Directed Lung Adenocarcinoma Cell Migration in a Micro-Controlled Environment

          • Thesis link

  • 2012 - 2014, thematic research under Dr. Cheng, Ji-Yen, Academia Sinica

          • Research Topic: Microfluidic cell culture platform

          • Research project: Whole cell sensing platform

  • 2014 - 2020, Graduate Research Assistant, Micro/Bio/Nanofluidics Unit in Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan

          • Topic: Automated microfluidic platform for cell biology and bioengineering research.

          • Involved Projects

          • Thesis title: Glioma-on-Chips: Analysis of glioma cell guidance and interaction in microfluidic-controlled microenvironments enabled by machine learning

          • [DOI:10.15102/1394.00001211]

          • Thesis opensource repository

  • 2017 - 2020, DC1 Research Fellow for JSPS, Micro/Bio/Nanofluidics Unit in Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan

          • Topic: Study of glioma infiltration in complex microenvironment on an automated microfluidic chip.

  • 2020.2 - 2020.3, PD Research Fellow for JSPS, Micro/Bio/Nanofluidics Unit in Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan

  • 2020.3 - 2020.7, Junior Research Fellow, Micro/Bio/Nanofluidics Unit in Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan

  • 2020.8 - 2021.12, Technology Pioneer Fellow, TDIC, Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan

Work experience

  • 2010 - 2010, Internship, National Taiwan University Hospital Clinical Laboratory, Taiwan

  • 2013 - 2014, Alternative Civilian Service in Ministry of Health and Welfare, Taiwan

  • 2014 - 2014, Research assistant to Dr. Cheng, Ji-Yen, Academia Sinica

  • 2020.3 - 2020.7, Junior Research Fellow, Okinawa Institute of Science and Technology, Okinawa, Japan

  • 2020.8 - 2021.12, Technology Pioneer Fellow, TDIC, Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan

  • 2021.09 - now, Founder, Ryudyn corporated limited, Taiwan

  • 2022.02 - now, Advisor, Ryudyn corporated limited, Taiwan

Fellowship, grant, award, and certificate

  • 2008 - 2009 Undergraduate Student Thematic Grant from National Science Council of Taiwan, Grant No.NSC97-2815-C-002-072-B

          • Grant topic: The Effect of Farnesol on the Lipase and Protease Activity of Staphylococcus aureus and Staphylococcus epidermidis. (TWD$48000)

  • 2008 National Taiwan University Department of Clinical Laboratory Science and Medical Biotechnology Theodore Memorial Scholarship

  • 2009 Research Creativity Award from the National Science Council of Taiwan.

  • Medical Technologist, Taiwan (2011)

  • 2012 National Yang-Ming University Annual Thesis Competition Excellent Award

  • Emergency Medical Technician-1, Taiwan (2014)

  • 2017 - 2020 Grands-in-Aid for Scientific Research (KAKENHI) to Research Fellowship for Young Scientists (DC1) from Japan Society for the Promotion of Science (JSPS), Japan

          • Grant topic: Study of glioma infiltration in complex microenvironment on an automated microfluidic chip. (17J00362) (¥3.1M)

  • 2020 - 2022 Proof-of-Concept Grant awarded to Technology Pioneer Fellow, OIST

Conference attendance

  • Oral Presentation, International Photonics Conference 2011 (IPC2011) (Dec.8-10, 2011, NCKU, Tainan, Taiwan)

  • Poster, The 20th Symposium on Recent Advances in Cellular and Molecular Biology (Feb.7-9, 2012, Kaohsiung, Taiwan)

  • Poster, The 17th Biophysics Conference, (May 23-25, 2012, Taipei, Taiwan)

  • Poster, The 4th International Symposium on Microchemistry and Microsystems (ISMM 2012), (Jun.10-13, 2012, Zhubei city, Hsinchu, Taiwan)

  • Poster, The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012), (Oct.28-Nov.1, 2012, Okinawa, Japan)

  • Poster, Advances in Microfluidics and Nanofluidics 2014 (AMN2014), (May, 2014, Academia Sinica, Taipei, Taiwan)

  • Technical presentation, NanoEngineering for Medicine and Biology 2016 (NEMB2016) (Feb, 2016, Houston, Texas, USA)

  • Poster presentation, Lab on chip world congress 2017. (Oct, 2017, California, USA)

  • Poster presentation, Lab on Chip & Microfluidics Europe 2019 (Jun, 2019, Rotterdam, The Netherlands)

Patent

  1. EP3350310B1 3D POLYMERIC INSERT TO APPLY UNIFORM ELECTRIC FIELD IN CIRCULAR CULTUREWARE

  2. US provisional application 62/968,768 A DETACHABLE MICROFLUIDIC SYSTEM AND METHOD FOR ROBUST SPARSE CELL SEEDING WITHOUT BUBBLES.

  3. US Provisional Patent Application No. 63/287,005 SYSTEM AND METHODS FOR OBJECT IMAGING, REAL-TIME ANALYSIS, AND PHENOTYPIC PREDICTION IN A MODULAR MICROSCOPE EMBODIMENT WITH INTERNET INTERACTIVITY


Publication

  1. Hsieh-Fu Tsai, Camilo IJspeert, Amy Q. Shen*, Voltage-gated ion channels mediate the electrotaxis of glioblastoma cells in a hybrid PMMA/PDMS microdevice, APL Bioengineering, 4, 036102 (2020)

    • [Original article]

    • [Featured article]

    • [DOI:10.1063/5.0004893]

    • [Emerging SCI, EI]

    • [Summary] By using a hybrid PMMA/PDMS microdevice to study the electrotaxis of glioblastoma. We identified that T98G and U-251MG glioblastoma cell lines has opposite electrotactic and electroalignment behavior. Also, the electrotaxis of glioblastoma cell lines depends on the laminin extracellular matrix, which in the brain is exclusively associated with the vasculature, suggesting a link to the peri-vascular microenvironment. The role of voltage gated calcium channels are further investigated in the electrotaxis of glioblastoma cells.

  2. Hsieh-Fu Tsai*, Kazumi Toda-Peters, Amy Q. Shen*, Glioblastoma adhesion in a quick-fit hybrid microdevice, Biomedical Microdevices, 21(2), 30, (2019)

    • [Original article]

    • [DOI:10.1007/s10544-019-0382-0]

    • [SCI, EI]

    • [Summary] A novel quick-fit hybrid PMMA/PDMS microdevice is developed and proved by studying a glioblastoma adhesion model. Over 150 V/m electric field, the electroalignment effect modulated the parallel alignment of the shear flow on the endothelial cells. We also observed the intercellular transport event suggesting that indeed cancer cells and fuse with endothelial cells.

  3. Hsieh-Fu Tsai*, Joanna Gajda, Tyler F.W. Sloan, Andrei Rares, Amy Q. Shen*, Usiigaci: Instance-aware cell tracking in stain-free phase contrast microscopy enabled by machine learning, SoftwareX, 9, 230-237 (2019)

    • [Original software article]

    • [DOI:10.1016/j.softx.2019.02.007]

    • [SCIE, EI]

    • Github repo

    • Youtube introduction video

    • [Summary] Usiigaci is an all-in-one Python-based cell segmentation, tracking, data analysis pipeline to analyze cell migration under stain-free phase contrast microscopy using machine learning methods (Mask R-CNN).

  4. Hsieh-Fu Tsai, Alen Trubelja, Amy Q. Shen* and Gang Bao*, Tumour-on-a-chip: microfluidic models of tumour morphology, growth and microenvironment, Journal of Royal Society Interface, 20170137 (2017)

    • [Review]

    • [DOI:10.1098/rsif.2017.0137]

    • [SCIE]

    • [Summary] Review some recent microfluidic platforms targeting research in tumor microenvironments and applications.

  5. Toshiaki Mochizuki, Yi-Jyun Luo, Hsieh-Fu Tsai, Akane Hagiwara and Ichiro Masai*, Cell division and cadherin-mediated adhesion regulate lens epithelial cell movement in zebrafish, Development, 144, 708-719 (2017)

    • [DOI:10.1242/dev.138909]

    • [SCIE]

    • [Summary] Investigation of cadherin and mechanical force in regulating lens epithelial cell movement during early development of zebrafish lens. I was involved specifically in the experiment of UV laser ablation and observe the immediate force induced cell migration after a wound.

  6. Joshua J. Cardiel, Daisuke Takagi, Hsieh-Fu Tsai and Amy Q. Shen*, Formation and flow behavior of micellar membranes in a T-shaped microchannel, Soft Matter, 12, 8226-8234 (2016)

    • [DOI:10.1039/C6SM01093H]

    • [SCIE, EI]

    • [Summary] Measurement and characterization of wormlike micelle membrane formation when interacting at the interface in the T-channel.

  7. Hsieh-Fu Tsai, Ji-Yen Cheng, Hui-Fang Chang, Tadashi Yamamoto, Amy Q. Shen*, Uniform electric field generation in circular multi-well culture plates using polymeric inserts, Scientific Reports, 6:26222 (2016)

    • [DOI:10.1038/srep26222]

    • [SCI]

    • [Summary] It is very difficult to create uniform electric fields from two electric potentials in a circular chamber due to differences of electric resistances. We employed a rational 3D computer aided design (CAD) approach of a microfluidic structure to equalize the electrical resistances in the chamber. A polymer insert with CAD structures can create uniform electric field in wells of multi-well plates or tissue culture dishes very much like a transwell insert. We hope this design of insert can be useful to push electric field stimultion into standard biomedical laboratory. Also the design strategy could be useful for different disciplines.

  8. Hsieh-Fu Tsai, Yi-Ching Tsai, Sharon Yagur-Kroll, Noa Palevsky, Shimshon Belkin, Ji-Yen Cheng*, Water pollutant monitoring by a whole cell array through lens-free detection on CCD, Lab on a Chip, 15, 1472-1480 (2015)

    • [DOI:10.1039/C4LC01189A]

    • [SCI,EI]

    • [Summary] Created a miniaturized platform for bioluminescent whole cell sensor using commercial linear charge-coupled devices.

  9. Hsien-San Hou, Hsieh-Fu Tsai, Hsien-Tai Chiu, and Ji-Yen Cheng*, Simultaneous chemical and electrical stimulation on lung cancer cells using a multichannel-dual-electric-field chip, Biomicrofluidics, 8, 052007 (2014)

  10. Kai-Yin Lo, Yun Zhu, Hsieh-Fu Tsai, and Yung-Shin Sun, Effects of shear stresses and antioxidant concentrations on the production of reactive oxygen species in lung cancer cells, Biomicrofluidics, 7, 064108 (2013)

  11. Hsieh-Fu Tsai, Ching-Wen Huang, Hui-Fang Chang, Jeremy J.W. Chen, Chau-Hwang Lee, Ji-Yen Cheng*, Evaluation of EGFR and RTK signaling in the electrotaxis of lung adenocarcinoma cells under dcEF stimulation, PLoS ONE, 8(8), e73418 (2013)

    • [DOI:10.1371/journal.pone.0073418]

    • [SCI]

    • [Summary] found that CL1-5 lung adenocarcinoma cell electrotaxis is independent of EGFR signalling despite CL1-5 cells express an abundant amount of EGFR. Suggesting the fundamental hypothesis may required modification.

  12. Hsieh-Fu Tsai, Shih-Wei Peng, Chun-Ying Wu, Hui-Fang Chang, Ji-Yen Cheng*, Electrotaxis of oral squamous cell carcinoma cells in a multiple-electric-field chip with uniform flow field, Biomicrofluidics, 6, 034116 (2012)

    • [DOI:10.1063/1.4749826]

    • [SCI EI]

    • Supplementary Information difficult to get from AIP, if supplementary information is needed please contact.

    • [Summary] multiple electric field with uniform flow field creation based on R-2R ladder network concept. Also found a easy method to achieve bubble free cell culture in thermoplastic microfluidic chips.

  13. Ji-Yen Cheng*, Mansoureh Z. Mousavi, Chun-Ying Wu, Hsieh-Fu Tsai, Blue light emission from a glass/liquid Interface for real-time monitoring of a laser-induced etching process, Journal of Micromechanics and Microengineering, 21, 075019 (2011)